Stemfit Weekend-free iPS/ES Cell Culture Media

Manufacturer: 
Nacalai Tesque, Inc
Specifications: 

 

 

StemFit® Basic Series by Ajinomoto: iPS/ES Cell Culture Media

High-Performance iPS / ES Cell Culture Media for Basic and Clinical Research

Under feeder-free culture conditions. StemFit®Basic02, Basic03, and Basic04 are capable:

Reprogramming, maintaining and differentiating human iPS and ES cells.

Features

  • Weekend-free feeding
  • Enabling single cell cloning
  • Providing high colony forming efficiency
  • Achieving high cost efficiency

Publication

Nakagawa, M. et al. (2014). A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells. Scientific Reports 4, Article number: 3594.

http://www.nature.com/articles/srep03594?message-global=remove&WT.ec_id=SREP-20140114

Materials provided

Component

Volume

Storage

Liquid A

400 ml

Store at below -20 °C

Liquid B

100 ml

Store at below -20 °C

Note: This product does not contain basic fibroblast growth factor (bFGF)

Media preparation

StemFit®Basic02 and Basic03 are provided frozen as a 2 component set containing “Liquid A” and “Liquid B” and can be stored at below -20 °C until use. Use sterile techniques to prepare StemFit® medium.

  1. Before use, thaw the frozen medium supplements at room temperature or overnight at 2-8 °C. Do not thaw the frozen supplements at 37 °C, as it accelerates the degradation of medium ingredients.
  2. Aseptically mix medium components by adding the full volume of “Liquid B” to “Liquid A”. Mix thoroughly. If prepared aseptically, StemFit® is ready for use.
  3. Upon thawing, StemFit®Basic02 medium may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit® may be stored at 2-8 °C for up to two weeks.
    Optional: We recommend storing the medium protected from light
  4. Before use, warm aliquots to room temperature and use immediately.

Highly stable and reproducible feeder-free culture system

Cells : Human iPS cell line 201B7 (seeding density listed in each experiment)

Surface: iMatrix-511

Medium: StemFit®Basic02

Passage technique: Single cell passaging with cell scraping, 0.5x TrypLE Select/EDTA

Easy transition from feeder-dependent to feeder-free culture

Feeder-dependent 201B7 iPSCs were transitioned to feeder-free conditions using respective cell adhesion substrates and seeded in StemFit®.

Superior performance

  • Superior growth performance on any matrices

  • Superior colony-forming efficiency from a single cell clone

 

Precaution and disclaimer

StemFit®Basic02 is for research use only and is not for diagnostic use, therapeutic use (including clinical research), or as a food. This product contains material derived from human plasma. Therefore, this product should be treated as potentially infectious agents and in accordance with universal handling procedures.

Referred protocols

Testimonials

Ordering Information

 
Product Cat.No. Storage Quantity
StemFit Basic02 BASIC02 -20°C Liquid A: 400 mL / Liquid B: 100 mL
StemFit Basic03 BASIC03 -20°C Liquid A: 400 mL / Liquid B: 100 mL
StemFit Basic04 (Coming soon) BASIC04 -20°C 500 mL