New products
Chromatrap® Your Chromatin!
Product Launch Promotions
KITS: Axil Scientific is giving away FREE TRIAL Chromatrap® kits to the first 6 customers
who indicate their interest here.
DISCOUNTS: In addition, any orders for Chromatrap® products placed from now till end March’18
will enjoy a special 5% discount.
Need further information, want to place an order, or have any questions at all? Contact our customer team at custcare@axilscientific.com.
---------------------------------------------------------------------------------------------------------------------------------------------------------
Chromatrap® Your Chromatin!
We are pleased to present you with Chromatrap®*, a revolutionary solid-state platform from the UK that enables more efficient, sensitive and robust chromatin immunoprecipitation (ChIP).
Compared to current methods, the following are what makes Chromatrap® superior:
Unlike bead-based ChIP, Chromatrap eliminates common manual handling problems and the need for time-consuming blocking steps to reduce non-specific binding. In under 5 hours from chromatin loading to qPCR or sequencing, Chromatrap significantly reduces the assay time for ChIP, enabling more samples to be analysed increasing laboratory throughput and efficiency.
- Highly sensitive – Perfect for low abundant proteins and transcription factors
- Low background – No pre-blocking required
- Reduced IP times – ChIP in under 5hrs
- Less manual handling – Reduced errors and sample loss
- Wide dynamic range – ChIP from as little as 1000 cells
- High throughput capability – Test multiple samples, antibodies and gene targets
- Cost-efficient solution – Save precious antibody
How does Chromatrap perform in the laboratory?
 |
|
| FASTER-SIMPLER-MORE SENSITIVE | COMPARISON TO BEADS |
|
|
|
|
|
|
|
|
|
|
|
Want to see Chromatrap®’s workflow? Check it out via their YouTube channel here.
Having trouble with ChIP or just getting started on ChIP? Check out the “Top 10 Tips for Positive ChIP Results” here.
About Chromatrap®: Porvair Sciences is part of Porvair plc, a specialist filtration company listed on the London Stock Exchange. They are one of the largest manufacturers of Ultra-Clean microplates, 96-Well Filter Plates and Microplate handling equipment for life science and synthetic chemistry, with over 25 years of experience in microplate manufacturing.
The potential of the patented filter used in Chromatrap® was first noticed by academics at Essex University and later developed in collaboration with Swansea University’s medical school.
In an academic/industrial partnership, the product range was developed to offer a faster, more sensitive method of chromatin immunoprecipitation. From an initial kit designed for PCR analysis, the technology has been developed into a full product range, particularly suited to small or low abundant biological samples.
The range is manufactured in Porvair’s bioscience facilities in North Wales and sold globally.
EpigenDx Offers Epic DNA Methylation Analysis Tools!
Product Launch Promotions
DISCOUNTS: In addition, any orders for EpigenDx products placed from now till end March’18
will enjoy a special 5% discount.
Need further information, want to place an order, or have any questions at all? Contact our customer team at custcare@axilscientific.com.
---------------------------------------------------------------------------------------------------------------------------------------------------------
Methylated genomic DNA controls: Intended to serve as positive or negative controls in DNA methylation analysis, and available for human, mouse, rat, and monkey studies.
Highly methylated DNA with greater than 85% methylation and low methylated DNA with lower than 5% methylation.
These are tested on numerous gene-specific and global methylation assays using pyrosequencing technology.
Validated Bisulfite Sequencing Primers: Over 7000+ Off-the-Shelf Validated Assays and Primer Sets for Bisulfite Sequencing, DNA Methylation Analysis and Mutation Analysis for Human, Mouse, Rat, Monkey and more.
Quality Assurance: Assay conditions are validated with quantitative Pyrosequencing methylation technology to ensure that methylated and unmethylated DNA are amplified at the same efficiency. Complete primer sets and assay conditions are provided, optimised for bisulfite sequencing and other DNA methylation applications such as COBRA and HRM real-time PCR.
Validated Methylation Assay Panels for Sequencing (available as Kits or Services): Currently available panels include FOX3, Cancer, Stem Cell, Immunology and BDNF Panels.
Custom Panel Option: Send in custom target regions with your genes of interest and receive a validated panel of assays tailor-made to your research interests.
About EpigenDx: EpigenDx, a company incorporated in 2006 in Massachusetts, US, is a genomic and epigenomic research company specializing in disease biomarker discovery, validation and molecular diagnosis, with an unmatched expertise in the field of DNA methylation analysis. EpigenDx has extensive knowledge and expertise in Pyrosequencing, targeted Next-Gen Sequencing, bisulfite sequencing, and various epigenomic research analysis techniques and methods stemming from their years of experience in the industry.
High Protein Yield - NEW: FectoPRO™
FectoPROTM gives amazing antibody production yields in suspension CHO cells.
Cells were seeded at 1 x 106 cells/ml in 30ml of FreestyleTM CHO Expression Medium and transfected with FectoPROTM (0.8μg DNA/ml) or FreeStyleTM MAX Reagent (1.25μg DNA/,ml)
following recommended protocols for the respective reagents. Recombinant mouse lgG production yield was assayed 1 to 6 days after transfection using protein G biosensor (fortéBIO® octet RED96 system).
Data kindly provided by ProteoGenix SAS (France)
FectoPROTM gives high transfection efficiency in suspension CHO cells.
FreeStyleTM CHO-S cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO Expression Medium and transfected with FectoPROTM + FectoPROTM Booster of FreeStyleTM MAX Reagent using 0.4μg/ml and 1.25μg/,l of a GFP expressing plasmid, respectively. GFP expression was assayed 24 h after transfection using fluorescence microscopy.
FectoPROTM gives superior protein prodcuction yields in suspension CHO and HEK-293 cells.
FreeStyleTM CHO-S and HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO or 293 Expression Medium and transfected with FectioPROTM + FectoPROTM Booster (0.4μg DNA/ml), FreeStyle TM MAx Reagent (1.25μg DNA/ml) or PEIproTM (1μg DNA/ml) following recommended protocols for the respective reagents. IgG3-Fc (34kDa) production was assayed 72 h (CHO cells) and 120 h (HEK-293 cells) after transfection by protein G affinity quantification (HPLC) and Western Blot. Standard corresponds to purified IgG3-Fc (34kDa).
FectoPROTM HEK-293F cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM 293 Expression Medium and transfected with FectoPROTM + FectoPROTM Booster (0.5 μg DNA/,l) following the standards protocol. IgG3-Fc production was assayed at different day by protein G affinity quantification (HPLC). Similar results are obtained with FreeStyleTM CHO-S (data not shown).
FectoPROTM is easily scalable and reproducible in CHO cells.
FreeStyleTM CHO-S cells were seeded at 1 x 106 cells/ml in 30ml, 100ml, 300ml or 1L of FreeStyleTM CHO Expression Medium and transfected with FectoPROTM + FectoPROTM Booster (0.5μg DNA/ml). At day 1 to 6, cell viability was assayed using Trypan blue exclusion assay (A) and IgG3-Fc production was assayed by protein G affinity quantification (HPLC) (B) and Western Blot (C). Standard (C) corresponds to purified IgG3-Fc (34 kDa).
Suspension CHO cells were seeded at 1 x 106 cells/ml in 30 ml of CHO medium and transfected using 3 different lots of FectoPROTM (CCIII 078, CCIII 104 or CCIII 106) following the standard protocol. 72 h after transfection, IgG3-Fc production was assayed by protein G affinity quantification. Similar results are obtained on suspension HEK-2933 cells (data not shown).
FectoPROTM gives superior protein production yields in suspension CHO cells grown in different media. CHO cells were seeded at 1 x 106 cells/ml in 30 ml of FreeStyleTM CHO Expression Medium, CD CHO Medium or Pro-CHOTM 4 Medium and transfected with FectoPROTM + FectPROTM Booster or FreeStyleTM MAX Reagent following recommended protocols for the respective reagents. IgG3-Fc production was assayed 72 h after transfection by protein G affinity quantification (HPLC).
Molecular Biology Reagents
1st BASE launches their range of Molecular Biology Reagents with new products and more product enhancements.
High Performance PCR Reagents
Product Information
|
Product No. |
Description |
|
BIO-5110-500U |
Taq DNA Polymerase (recombinant), 500U |
|
BIO-5120-1ml |
dNTP Mix, 10mM each, 1.0ml |
|
BIO-5125-1ml |
dNTP Set, 100mM, 4 X 0.25ml |
Products for DNA Electrophoresis
Product Information
|
Product No. |
Description |
|
BIO-5130-50ug |
100bp DNA Ladder, Ready-To-Use, 50ug |
|
BIO-5130-100ug |
100bp DNA Ladder, Ready-To-Use, 100ug |
|
BIO-5140-50ug |
1kb DNA Ladder, Ready-To-Use, 50ug |
|
BIO-5140-100ug |
1kb DNA Ladder, Ready-To-Use, 100ug |
|
BIO-5160-6ml |
6X DNA Loading Dye, 3-Colour, 6 X 1ml |
|
BIO-5170-1ml |
DNA Stain, 1ml |
Prestained Protein Ladder
Broad range protein ladder with an Orange reference band at ~75kDa and a Green reference band at ~25kDa
Product Information
|
Product No. |
Description |
|
BIO-5150-250ul |
Prestained Protein Ladder, 3-Colour, 250ul |
|
BIO-5150-500ul |
Prestained Protein Ladder, 3-Colour, 500ul |
Nanovex Pronanosome Series
View the Nanovex Catalogue here.
Our standard Pronanosomes are formulated to obtain niosomes or liposomes able to encapsulate different
compounds and to be used in multiple applications.
Our intracellular delivery Pronanosomes are formulated to obtain niosomes or liposomes able
to deliver the encapsulated drug intracellularly.
Our controlled delivery Pronanosomes are formulated to obtain nanovesicles able to control the
delivery of the encapsulated drug with the temperature or pH.
Our Pronanosomes BBB and dermal are formulated to obtain nanovesicles able to vehiculate
compounds through the BBB or through the skin, respectively.
All our Pronanosomes can be ordered with other extra characteristics shown above (From 1 to 4 simultaneously) to suit the needs of our customers.
| All our Pronanosomes can be ordered with other extra characteristics shown above (From 1 to 4 simultaneously) to suit the needs of our customers. |  |
Newly Added Growth Factors
Growth factors and stem cell reagents have an important role in cancer and developmental research. GoldBio’s growth factors have continued to explode on the marketplace, which is why they are so excited to announce that they are expanding this product line! Currently, more than 100 new growth factors have been added to their pre-existing list, and they plan to add more throughout the year.So stay tuned for more great offers!
They have added new EGFs, PDGFs, Cytokines, FGFs, Interleukins, and Neurotrophic factors as well as a completely new category: Interferons. These additions were based on your feedback and requests and they’re excited that they can provide you with even more of the products they need at the same great rates that GoldBio has been providing for years!
ALSO SEE THESE EXPANDED OR NEW CATEGORIES:
FGFS
The FGF family of proteins is one of the largest polypeptide growth factor families in the animal kingdom, found in organisms ranging from nematodes to humans. FGFs are involved with embryonic development, angiogenesis, wound healing, metabolic regulation and cancer. GoldBio’s selection of growth factors includes 25 FGFs, with the line continuing to grow. Among the newly added FGFs are FGF4, FGF13, FGF19 and FGF21.
INTERLEUKINS
Interleukins are a large family of cytokines which are soluble hormone-like mediators of the immune system. They involved in processes of cell activation, cell differentiation, proliferation and cell-to-cell interactions. The expression of interleukins is strictly regulated. For example, the factors are often not secreted constitutively. GoldBio offers a large catalog of interleukins, with new additions: IL1A, IL1RN, IL2 Cys141Ser, IL5, IL8, IL9,IL17B, IL21 and IL36B.
NEUROTROPHIC FACTORS
Neurotrophic factors are involved in many roles of the CNS beyond growth and development. They are responsible for the maintenance and survival of neurons and have been the subject of extensive research. There is continued interest in the research of potential neurotrophic factor therapies, which may help treat Alzheimer’s disease, Parkinson’s disease and other neurodegenerative disorders. GoldBio offers high-quality recombinant neurotrophic factors. Our newest additions to the line include CDNF, CNTF, GMFB, MANF, MDK, NRG1-alpha, NRG1-beta1, NRG1-beta2, NRN1, PSPN and PTN.
INTERFERONS (NEW CATEGORY)
Interferons are a family of cytokine proteins that play an important role in modulating immune response. They are secreted in response to viruses and other pathogens, triggering such responses that prevent viral replication, activation immune cells, inhibit cell division and affect cell differentiation. There are three classes of interferons: Type I, Type II and Type III IFN which relate to the type of receptor through which the IFN signal. GoldBio is excited to offer a variety of each IFN of each type, including: IFN-alpha1A, IFN-alpha1B, IFN-alpha2A, IFN-alpha2B, IFN-beta1B, IFN-gamma, IFN-lambda1, IFN-lambda2, IFN-lambda3 and IFN-omega1.
Smooth Muscle Cells (7 Types)
Lifeline Now has 7 Different Types of Normal Human Smooth Muscle Cells!
Lifeline’s Smooth Muscle Cells and VascuLife SMC Media have been cited in 12 recent publications.
See our Reference Page for more information
|
Get better results with Lifeline's Superior Normal Human Smooth Muscle Cells Lifeline’s Smooth Muscle Cells are isolated from the Aorta, the Pulmonary Artery, the Coronary Artery, from Bronchial Tracheal tissue, from the Lungs, from the Bladder, and from the Uterus. Each smooth muscle cell product demonstrates excellent morphology and growth in our fully optimized VascuLife SMC Media |
When Lifeline's Human Smooth Muscle Cells are grown in VascuLife SMC optimized medium, our cell growth far exceeds the competition. Lifeline's Human Smooth Muscle Cell Systems provide an ideal low serum (5%) culture system, ideal for the study of: Angiogenesis; Atherosclerosis; Diabetes; Pulmonary Arterial Hypertension; Asthma; and Bladder Disorders and Uterine Contractibility Studies
| Lifeline's Smooth Muscle Cell Systems |
| Human Uterine Smooth Muscle Cell Systems, (HUtSMC) |
| Human Aortic Smooth Muscle Cells, (HAoSMC) |
| Human Pulmonary Artery Smooth Muscle Cells, (HPASMC) |
| Human Coronary Artery Smooth Muscle Cells, (HCASMC) |
| Human Lung Smooth Muscle Cells, (HLSMC) |
| Bronchial/Tracheal Smooth Muscle Cells, (HBTSMC) |
| Human Bladder Smooth Muscle Cells, (HBSMC) |
| VascuLife SMC Complete Kit |
| FrostaLife Cryopreservation Solution ; 100mL |
| TrypKit , 100mL |
