cDNA synthesis kits

Toyobo has various kits for cDNA synthesis based on the genetically improved M-MLV reverse transcriptase "ReverTra Ace^®". ReverTra Ace -α-^® is an efficient and convenient kit to synthesize high quality cDNA. This kit contains the highly efficient reverse transcriptase "ReverTra Ace^®", as well as other components optimized for the synthesis of long cDNAs suitable for RT-PCR.
ReverTra Ace^® qPCR RT Kit (Code No. FSQ-101) is an efficient and convenient kit to synthesize high quality cDNAs for real-time PCR. This kit contains the highly efficient reverse transcriptase "ReverTra Ace^®" and a RT buffer optimized for the highly efficient synthesis of short-chain cDNAs suitable for real-time PCR. ReverTra Ace^® qPCR Master Mix (Code No. FSQ-201) is a premix version of the ReverTra Ace^® qPCR RT Kit.
ReverTra Ace^® qPCR RT Master Mix with gDNA remover (Code No. FSQ-301) is an efficient and convenient kit, consisting of master mix reagents, to synthesize high quality cDNAs for real-time PCR. The kit includes reagents for reverse transcription and for the removal of genomic DNA. The protocol consists of i) a genomic DNA degradation step using "gDNA remover [DNase I]" and ii) a reverse transcription step. The two steps can be achieved sequentially without purification or heat inactivation of DNase I.

cDNA synthesis Kits

Enzyme	Product Name	Application	Efficiency	Long cDNA synthesis	Master Mix	Genomic DNA removal	Recommend PCR enzymes
ReverTra Ace®	ReverTra Ace -α-®	Conventional RT-PCR	+	++ (> 10 kb)			KOD –Plus- series KOD FX series Taq-based PCR reagent
	ReverTra Ace® qPCR RT Kit	qPCR	++	+ (< 5 kb)			THUNDERBIRD® series Realtime PCR Master Mix series KOD SYBR® qPCR Mix KOD –Plus- series KOD FX series Taq-based PCR reagent
	ReverTra Ace® qPCR RT Master Mix	qPCR	++	+ (< 5 kb)
	ReverTra Ace® qPCR RT Master Mix with gDNA Remover	qPCR	++	+ (< 5 kb)
	SuperPrep® Cell Lysis & RT Kit for qPCR	qPCR from cultured cells	++	+ (< 5 kb)

++: Excellent, +: Good, : Applicable