Yikon Genomics
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Specifications:
MALBAC Single Cell WGA Kit
Whole Genome Amplification for Single Cells or Minor Genome
☞ Quasilinear amplification with least amplification bias
☞ Reproducible representation including consistent locus amplification in both AT- and GC- region
☞ High Coverage that <10% locus drop-out
☞ Rapid Procedure with 1 tube, 3 steps and 4 hours
☞ Application for CNV&SNV analysis, PGS/PGD, CTC, etc.Products Cat. # MALBAC Single Cell WGA Kit (10 rxns)
Cell Lysis Buffer, Cell Lysis Enzyme, Pre-Amp Buffer, Pre-Amp Enzyme, Amp Buffer and Amp Enzyme for 10 reactions.
YK001A MALBAC Single Cell WGA Kit (50 rxns)
Cell Lysis Buffer, Cell Lysis Enzyme, Pre-Amp Buffer, Pre-Amp Enzyme, Amp Buffer and Amp Enzyme for 50 reactions.
YK001B Overview
MALBAC Single Cell WGA Kit is based on the novel patented MALBAC technology, and provides rapid, reliable and reproducible whole genome amplification from single cells or equivalent gDNA (picogram level) to produce 2-4 micrograms of amplified gDNA in about 4 hours. The kit is able to achieve >90% amplification success rate and consistent amplification efficiency in both AT- and GC-rich regions for most cell types, such as single blastomere, polar bodies, single sperm and single cells from cultured cell lines. The kit generates reproducible loci representation, achieving>0.9 correlation coefficient for Qpcr Ct values among reaction replicates. More than 90% of genetic loci over a genome can be amplified then analyzed by various plat forms, such as real-time PCR (qPCR) and next-generation sequencing. The kit can also be used to amplify sub-nanogram level of inputting g DNA (e.g. from hundreds of cells).
Workflow
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Specifications:
MALBAC Single Cell WGA Re-Amp Kit Re-Amp
efficiently from 1-10 ng MALBAC products
☞ 1-3 ug gDNA can be amplified from 1-10 ng MALBAC products within 1 hour.
☞ The Re-Amped products have consistent locus representation compared with the original MALBAC products.Products Cat. # MALBAC Single Cell WGA Re-Amp Kit (10 rxns)
Re-Amp Buffer and Re-Amp Enzyme Mix for 10 reactions.
YK012A MALBAC Single Cell WGA Re-Amp Kit (50 rxns)
Re-Amp Buffer and Re-Amp Enzyme Mix for 50 reactions.
YK012B Overview
MALBAC Single Cell WGA Re-Amp Kit is a companion product of MALBAC Single Cell WGA Kit. By this Re-Amp Kit, one can obtain ~1-3 µg gDNA from 1-10 ng previous MALBAC amplified products in about 1 hour. The Re-Amped products keep good fidelity to the first round MALBAC products without introducing further amplification bias. The Re-Amped products can also be applied for various downstream experiments, such as real time qPCR, Sanger sequencing (point mutation detection), next generation sequencing, etc.Workflow
FAQs
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Specifications:
Single Cell Lysis Kit
Lysing cells completely in a low salt buffer
☞ Completely lyses cells in low concentration of Ca2+, Mg2+
☞ Efficiently lyses cells in 1 hour
☞ The lysed cells can be applied for MALBAC amplification, gDNA extraction, or directly for real time qPCR, etc.Products Cat. # Single Cell Lysis Kit (10 rxns)
Cell Lysis Enzyme and Cell Lysis Buffer for 10 reactions.
YK011A Single Cell Lysis Kit (50 rxns)
Cell Lysis Enzyme and Cell Lysis Buffer for 50 reactions.
YK011B Overview
Single Cell Lysis Kit is to lyse cells completely and release gDNA into a low salt buffer. Unnecessary for any purification, the low salt lysed products can be directly used for various downstream applications such as whole genome amplification and real-time qPCR. About 1 to 1000 mammalian cells can be efficiently lysed in a standard lysing reaction. This lysis kit can be applied to freshly cultured cells, FAC cells, laser capture microdissection, diluted cells, micromanipulated samples and biopsy samples, etc.
Workflow
